Perkin-Elmer LS-55 Fluorimeter#
A fluorimeter measures the fluorescence or light emitted by different fluorescing objects. Fluorescence occurs when light of specific wavelength hits and excites electrons in a sample, and the electrons in that sample instantly emit or fluoresce light of a different wavelength. Typically, molecules with conjugated and/or aromatic systems exhibit fluorescence. Ideally, emission and excitation spectra for the same analyte are mirror images of eachother with the excitation wavelenght being shorter than the emission wavelenght. In fluorescence spectroscopy, the light source and the detector are oriented at a 90 degree angle. Unlike UV-Vis spectroscopy, fluorescence spectroscopy is a measure of the ability of an analyte to emit light.