Fixed Pathlength Cell Analysis ============================== #. Similarly, you will run your samples again using a fixed pathlength cell to determine which method is better at analyzing your samples #. Using a plastic syringe, you will inject your sample into the cell (it may overflow slightly) #. Following the sample procedure above, you will run each of your samples, flushing the fixed cell with methylene chloride in between runs. .. tip:: Starting with the least concentrated sample and working up to the most concentrated sample will greatly increase the accuracy of the data for fixed salt plates. #. Once all of your scans has finished you can analyze your data similar to the steps provided above when using the salt plates.