PURINE METABOLISM STUDIES

GUANINE AMINOHYDROLASE; IOSLATION AND PURIFICATION BY AFFINITY CHROMATOGRAPHY

INOSINE MONOPHOSPHATE DEHYDROGENASE; ISOLATION AND PURIFICATION BY A DOUBLE AFFINITY CHROMATOGRAPHY METHOD

SEA URCHIN PROTEOLYTIC ENZYME STUDIES

ISOLATION AND PURIFICATION

FLUORESCENT GEL SLICE ASSAYS

BAY SCALLOP MICROSATTELITE GENETIC STUDIES

ISOLATION OF GENOMIC DNA FROM INDIVIDUAL SCALLOPS

BLUNT END RESTRICTION ENDONUCLEASE DIGESTIONS WITH Nhe I, Alu I, Rsa I, and Hae III

LIGATION WITH UNIVERSAL LINKER SNX

SUBTRACTIVE HYBRIDIZATION WITH 5' BIOTINYLATED REPEAT OLIGONUCLEOTIDES WITH STREPTAVIDIN-COATED MAGNETIC BEADS

PCR AMPLIFICATION OF REPEAT-ENRICHED DNA

CUTTING OF SNX UNIVERSAL LINKER WITH Nhe I and CUTTING OF PROMEGA pGEM -3Z PLASMID WITH Xba I

LIGATION OF Nhe I CUT REPEAT-ENRICHED DNA INTO Xba I CUT PLASMID

CLONING OF PCR AMPLIFIED REPEAT-ENRICHED DNA

DNA SEQUENCING OF CLONED AMPLIFIED REPEAT-ENRICHED DNA

Bay Scallop Microsattelite Results

• REN Digestion
• PCR REN Digest Results
• Scallop 1 dilution
• Scallop 2 dilution
• Scallop 3 dilution
• SNX PCR ligation check
• Nhe I digested SNX PCR ligation check
• Shotgun Oligo PCR results
• Oligo PCR gel 1 results
• Oligo PCR gel 2 results
• Scallop 1 with various GTC oligos
• Scallop 2 with various GTC oligos
• Scallop 3 with various GTC oligos
• SNX PCR ligation check
• 18S biotinylated probe check
• 10/14 SNX PCR ligation check for several REN digestions
• Subtractive hybridization results with Duncan's biotinylated oligos
• Xba I digestion of pGEM -3Z and not pGEM -5Zf(+)
• Plasmid ligation check using M13/pUC primers -47 forward and -48 reverse
• (GTC)5 Oligo PCR with 9 Scallop Individuals
• Three PCR primer pairs with 1 scallop individual 10/8/03