LABS
Gradual Notebook Module 1-1
Gels:
UVP00362,
UVP00363, UVP00364,
Destained overnight
Gradual Notebook Module
1-2
Gradual Notebook Module 1-4
Gradual Notebook Module 1-5
Determining Fragment Size Using Excel (revised)
Gels from 2/19/08
UV000367,
UV000369, UV000370,
UV000371,
UV000372
I don't think it will be possible to determine size from any of
these. So, use the diagrams hyperlinked below. Measuring from the front edge of
the wells and bands, determine the size of the bands in lanes 3-6 and the
orientation of the inserts in the two diagrams. Include your analysis in your
lab notebook. Also include picture(s) of your group's gel and reasonable explanations for the results you obtained.
Diagram 1
Diagram 2
NOTE: On Tuesday, 3/5/08, you will repeat the PCR lab from 2/26/08. Have the procedures written out and tables filled in. Carefully read the protocol and include the 1kb ladder in your gel.
NOTE: the new due date for the PCR notebook
section is Tuesday 3/25/08. I'll be posting a set of questions to be
included in your hand-in. We will begin Project 3A (below) on Tuesday 3/18/08.
QUESTIONS FOR PCR LAB
PROJECT 3A----Purification of RNA from bee larvae
PROJECT 3b----Purification of DNA and Protein from bee larvae (3/25/08)
RT/PCR and cloning of cDNA from RNA
Manual-- Transcriptor First
Strand Synthesis Kit
RT Protocol for
3/25/08
PCR/Ligation reaction for
3-25-08
PROJECT 3c----Quantitation of Protein and
SDS-PAGE
Quantitation of Protein
SDS-PAGE
Transfer of Proteins to
Membrane
Protein Assay 11/15/06
Project 3d-----Western Blot Analysis
PROJECT 5---SEQUENCING AND BIOINFORMATICS
Protocol Page 1
Protocol Page 2
PROJECT 5--BIOINFORMATICS WEBSITES
National Center for
Biotechnological Information-Homepage
Sequence
Massager
VecScreen
BLAST
ORF Finder
ExPASy Translation Tool
