Dr. Candice M. Klingerman's Research Laboratory

Thank you for visiting my laboratory's webpage. We are a small research lab located in the Hartline Science Center at Bloomsburg University. Myself, along with my students and collaborators, are dedicated to understanding diseases of energy dysregulation. My research is fluid and constantly evolving so continue to check back for updates.

 

Research Interests

 

A. Fertility and Energy Dysregulation

My laboratory's primary research area is the link between energy balance and reproduction in male and female mammals.  Trying to understand and treat diseases of energy dysregulation, i.e. obesity and anorexia, is difficult and has been misunderstood for decades.  Instead, we link energy balance to another evolutionary process, reproductive success. Reproductive success is based upon the concept that any adaptive trait that mammals have developed, such as the ability to carry very high levels of subcutaneous and intra-abdominal fat, did so because they increased the probability of a successful mating. 

       

Hamster pictures taken by Zubair Kadr in Professor Jill Schneider's laboratory (Lehigh University).

 

Specifically, I am studying why it is that female, Syrian hamsters (Mesocricetus auratus) in negative energy balance (e.g. anorexia) increase the motivation for food (i.e. food hoarding) and decrease the motivation to reproduce (i.e. scent marking) but not food intake or sexual performance (i.e. lordosis reflex).  Current methodologies involve behavioral observation, immunohistochemical staining of brain regions responsible for mediating appetite and sexual behavior, and hormone assays. 

 

Klingerman Energy Balance Publications

Sense and nonsense in metabolic control of reproduction

Energetic challenges unmask the role of ovarian hormones in orchestrating ingestive and sex behaviors

Food restriction dissociates sexual motivation, sexual performance, and the rewarding consequences of copulation in female Syrian hamsters

Cellular activation in gonadotropin-inhibiting hormone-immunoreactive cells is associated with sexual motivation and food hoarding, but not sexual performance and food intake in female Syrian hamsters

 

B. Hydrogen Sulfide Intoxication

My laboratory's secondary research area of interest is understanding how the toxic gas, hydrogen sulfide, induces its deadly affects and developing successful antidotes that reduce its toxicity and increase the chance of survival. Hydrogen sulfide is a problem in the gas and petroleum industry and mining and other natural events such the eruption of a volcano and the aggregation of algaes in the water.  In fact, the toxicity hydrogen sulfide (H2S) makes it second only to carbon monoxide for the number of gas-related deaths reported by the U.S. Bureau of Labor and Statistics (BLS.gov).  It is not clearly understood how H2S exerts its toxicity and therefore, no suitable antidote has been developed.  Two signs of major toxicity include a hyperventilation followed by a fatal apnea.  In patients who survive these stages, many report severe migrane headaches and anterograde amnesia. In collaboration with Philippe Haouzi, M.D., Ph.D. (University of Penn State College of Medicine, Hershey, PA), I am researching the mechanism of action of H2S using those respiratory symptoms as a guide, and testing various potential antidotes against the acute and chronic effects of H2S.

Disruption of the balance between plankton and bacteria that reduce sulfate. As a result, hydrogen sulfide gas is released in quantities large enough to kill human beings (picture courtesy of listverse.com)

 

Klingerman Hydrogen Sulfide Publications

Fate of intracellular H2S/HS− and metallo-proteins

H2S concentrations in the arterial blood during H2S administration in relation
to its toxicity and effects on breathing

 

Other Klingerman Publications

Second generation antipsychotics cause a rapid switch to fat oxidation that is required for survival in C57BL/6J mice

An evaluation of exogenous enzymes with amylolytic activity for dairy cows

The effect of Lactobacillus buchneri 40788 or Lactobacillus plantarum MTD-1 on the fermentation and aerobic stability of corn silages ensiled at two dry matter contents

The effect of wide swathing on wilting times and nutritive value of alfalfa haylage